Hybridoma/Screening

Overview of Services

The Hybridoma/Screening Core Facility was established to provide investigators at the Lerner Research Institute with a resource for monoclonal antibody production. The facility also provides consultation services to assist the investigator in all aspects of monoclonal antibody production. The services are performed under strict QC guidelines appropriate for antibodies that may have commercial potential.  

There are two phases of monoclonal antibody production: the development of the hybridoma cell line and production of the monoclonal antibody from the established hybridoma cell line. Currently, the HCF offers two methods for monoclonal antibody production from hybridoma cell lines: tissue culture for small scale amounts(mgs) and Integra Flasks for large scale amounts(grams). Polyclonal antibody production is offered on a limited basis, dependent on available space in the BRU. Finally, both protein G, A and peptide affinity purification are also performed in the core at small and large scale production levels.

The Core also offers Sigma Mission shRNA, CRISPR and LentiVirus clones at a discounted rate.


For information on antigen preparation for monoclonal and polyclonal antibody production please call Melanie Hoffner. Feedback and suggestions: Email hoffnem@ccf.org , Phone (216) 445-0769

To order shRNA clones:

1. Check our in-house shRNA collection of available clones with controls: LRI shRNA CLONE LIST
2. If your clones are available, please request In-house clones.
3. If clones are not available please visit the Sigma website
4. Request Sigma clones
5. To order Lentivirus, visit the Sigma website and complete the shRNA Lentivirus Order Form, email shRNA@ccf.org for custom quote.

Leadership

Melanie Hoffner
Shared Laboratory Resource Specialist II
216-445-0769
hoffnem@ccf.org

Location and hours of operation

Links and Resources

1. Hybridoma Core Website

Contacts

Map